Figure 5
From: A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts
Isolation of protoplasts from green tissue. Protoplasts were prepared from 2-week-old kitaake plants and transformed as described (Methods). Picture was taken under bright field light using a Zeiss Axiovert 25 microscope with a 40× objective. Larger clear cells are derived from stem tissue whereas smaller chloroplast-filled cells were derived from green leaf tissue.