Fig. 2

Details of image analysis process used in the PYM procedure, from source to segmented image (a–d). a Source image taken with the infrared camera and the blue filter. b Grey image based on NEW CHANNEL values (after a crop of a image). c False 2 colour image for visual assessment of segmentation between plant and background. d Final image after segmentation, erosion, opening and closing procedures. White pixels are counted and summed to estimate projected leaf area