Skip to main content
Fig. 2 | Plant Methods

Fig. 2

From: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i

Fig. 2

Detailed process to obtain transformed grapevine embryogenic cells (cv. Chardonnay) and regenerated plantlets. Antibiotics selection for transformed cells allows to discern resistant white embryogenic cells (c, red outlined) and non-resistant black embryogenic cells (c, green outlined). (a-h) Indicate the different steps, directly described in this figure, of the plant method developped to obtain high frequency biallelic homozygous knock-out grapevine plantlets. BF: Brightfield. Figure created with biorender.com

Back to article page

Plant Methods

ISSN: 1746-4811