Fig. 3

Integrated analysis of gene structure, phylogeny, BLAST parameters, and domain protein prediction. The panel displays a schematic phylogenetic tree, demonstrating the evolutionary relationships among paralog genes. Additionally, it provides a visual representation of gene structures, highlighting exons (blue boxes), introns (connecting lines), and untranslated regions (UTR) (green boxes). This depiction allows for a quick assessment of the genomic organization of the gene of interest. In the right panel, a schematic outlines the parameters used in the BLAST (Basic Local Alignment Search Tool) analysis. Parameters such as sequence identity cutoffs, e-values, and coverage significantly impact the outcomes of homology searches, influencing the identification of related gene copies. Also included was the domain protein prediction, which illustrates identified protein domains