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Fig. 6 | Plant Methods

Fig. 6

From: 3D-CNN detection of systemic symptoms induced by different Potexvirus infections in four Nicotiana benthamiana genotypes using leaf hyperspectral imaging

Fig. 6

Northern blot hybridization analysis of total RNA extracts collected from four N. benthamiana (wt, DCL2/4, NahG, and AGO2) genotypes. Plants were mechanically-inoculated with 0.1M Tris-borate buffer (mock) or purified virions of one of the following potexviruses: Pepino mosaic virus (PepMV) SP13 (mild isolate), PepMV PCH 06/104 (severe isolate), Bamboo mosaic virus (BaMV) and Potato virus X (PVX). Total RNA extracts (1 to 5 μgr) from systemic leaves of mock- or virus-inoculated plants were collected and analyzed on northern blots hybridized with the respective DIG-labeled negative (–) strand coat protein probes

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Plant Methods

ISSN: 1746-4811