Fig. 1

A) Physiological changes produced in the cells as a consequence of culturing the plants in darkness. B) Micropropagated ‘Ca-PPV’ shoots grown 4 weeks with a photoperiod versus 8 weeks in the dark (etiolated). C) Effect of high temperature exposure on micropropagated ’Ca-PPV’ shoots 30, 35, 40 and 45 days. Survival decreases with exposure time whereas probability of finding pathogen-free plants should increase