Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings

Table 5 Effects of antibiotic treatment on in vitro establishment

Antibiotics	N	Fungal contamination		Bacterial contamination		Shoot formation rate		Shoots per explant (median ± IQR)		Shoot length (median ± IQR)
Antibiotics	N	%	p value	%	p value	%	p value	N	p value	[mm]	p value
Not added	17	0	1.000	41	< 0.001	94	0.019	1.0 (± 1.00)	0.608	6.0 (± 4.75)	0.231
Added	34	3	1.000	0	< 0.001	62	0.019	1.0 (± 1.00)	0.608	5.0 (± 4.00)	0.231

Cefotaxime (500 mg/L), gentamicin (50 mg/L), and streptomycin (50 mg/L) were either added or not added during sterilization and in the medium. The evaluation was performed after 12 weeks of in vitro culture. The sample size (N) of shoot tips grown on propagation media supplemented with or without 2% sucrose was ± 1. Differences between “not added” and “added” were analyzed by Fisher’s exact test for contamination and shoot formation rates or the Mann‒Whitney U test for the number of shoots and length
IQR, interquartile range

ISSN: 1746-4811