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Table 6 Effects of sucrose in propagation media on contamination and shoot formation in vitro

From: Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings

Medium

N

Fungal contamination %

Bacterial contamination %

Shoot formation rate %

Shoots per explant (median ± IQR)

Shoot length [mm] (median ± IQR)

M2 + 

26

0

8

73

1.0 (± 1.00)

5.5 (± 3.25)

M2 + woS

25

4

20

72

1.0 (± 0.50)

5.0 (± 5.50)

  1. The evaluation was performed 12 weeks after the introduction into in vitro conditions. The sample sizes (N) of shoot tips from Lower Saxony or Brandenburg treated with or without antibiotics were ± 1 between the groups grown on M2 + media supplemented with or without 2% sucrose (M2 + woS). According to Fisher’s exact test, no significant differences were identified for any of the parameters. IQR – interquartile range
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Plant Methods

ISSN: 1746-4811