Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings

Table 7 Surviving beech genotypes obtained in Exp I-III after being cultured up to 2 years

Experiment	Age	Provenance	N genotypes	Medium	Sugar source	Light intensity [µmol m⁻² s⁻¹]
I	14 years	19 (PL)	1	M2+ G	glucose	30
II	3 weeks	Grosshansdorf (DE)	10	M2+ woS	sugar-free	70
III	3 weeks	81009 (DE)	13	M2+ G	glucose	30
III	3 weeks	81009 (DE)	2	M2+ woS	sugar-free	70

Cultures were maintained under standard PGC conditions with an subcultivation period of 6 weeks on M2+ medium. M2+ medium contained either no sugar source (sugar-free—M2+ woS) or 2% glucose (M2+ G)

ISSN: 1746-4811