Fig. 5
From: Genetic transformation of the oilseed crop camelina using immature zygotic embryos
Confirmation of putative T1 transgenic plants by PCR using GFP-specific primers. Lanes 1–10: PCR-amplicons of randomly chosen T1 siblings derived from primary transgenic plant P24 of cv. Calena. Like in the wild-type (WT) control plant, no GFP-specific amplicon was obtained from plants T1-1 and T1-5 indicating them to be null segregants, whereas all other tested T1 siblings proved PCR-positive indicating their transgenicity. P: plasmid DNA, N: negative control (Master Mix without DNA), M: 100 bp DNA Ladder (Thermo Fisher Scientific), the expected position for 541 bp GFP-specific amplicons is indicated