Fig. 6

Evaluation of transgene expression and segregation of T₁ siblings derived from cv. Ligena T₀ plant P5 that carried a constitutively expressed GFP gene in hemizygous condition. Left: Out of the 20 randomly chosen T₁ siblings shown, five null segregants show bright red fluorescence in their above-ground parts due to the chlorophyll content, while their roots are hardly visible (white arrowheads) owing to the missing GFP gene. By contrast, the 14 GFP-transgenic siblings show bright green fluorescence in their roots, with the hypocotyls and cotyledons of particularly strong GFP-expressors (possibly homozygous transgenic segregants) appearing in orange color (orange arrowheads) owing to the mixture of light emitted by chlorophyll (red) and GFP (green). One seed (upper row, far left, grey arrowhead) did not germinate and hence was not considered in the segregation analysis. The image was taken by a Canon EOS M200 camera. Right: Representative image of the root elongation zone of a transgenic sibling captured by confocal laser scanning microscopy. Both images were taken after four days of germination upon excitation by monochromatic light at 488 nm and the use of GFP-specific filter sets