Table 11 Composition of standard culture media used to generate Transgenic camelina plants
From: Genetic transformation of the oilseed crop camelina using immature zygotic embryos
Components | Pre-culture medium | Inoculation medium | Co-cultivation medium | Shoot initiation medium | Shoot elongation medium | Rooting medium |
|---|---|---|---|---|---|---|
MS minerals, NH4NO3-free | 1x | 1x | 1x | 1x | 1x | 0.5x |
NH4NO3 (mM) | 5 | 5 | 5 | 5 | 5 | 2.5 |
B5 vitamins | 1x | 1x | 1x | 1x | 1x | 1x |
Sucrose (g/L) | 30 | 30 | 30 | 30 | 20 | 10 |
IAA (mg/L) | 2 | - | 2 | 2 | 0.1 | 0.1 |
Acetosyringone (µM) | - | 150 | 150 | - | - | - |
Hygromycin (mg/L) | - | - | - | 2 weeks 25 | - | - |
Timentin (mg/L) | - | - | - | 150 | 150 | 150 |
Phytagel (g/L) | 4 | - | 4 | 4 | 4 | 4 |
pH | 5.7 | 5.7 | 5.7 | 5.7 | 5.7 | 5.7 |