Genetic transformation of the oilseed crop camelina using immature zygotic embryos

Table 2 Effect of pre-cultivation period and time point of pricking of immature zygotic embryos on transformation efficiency

No. of IZEs¹	Duration of pre-cultivation (before inoculation)	Time point of pricking	No. of regenerated plantlets	No. of PCR- positive plants and efficiency²
60	2 days	after 1 day of pre-cultivation	6	3 (5%)
60	2 days	after 2 days of pre-cultivation	14	5 (8%)
60	3 days	after 2 days of pre-cultivation	8	2 (3%)
60	3 days	after 3 days of pre-cultivation	22	7 (12%)
60	3 days (control without inoculation)	after 3 days of pre-cultivation	0	0 (0%)

¹20 immature zygotic embryos (IZEs) of line Cam139 were cultivated per culture dish, with three dishes being used as repetitions per treatment
²PCR was performed using primers specifically amplifying the GFP transgene, and the transformation efficiency was calculated as proportion of agro-inoculated explants giving rise to GFP-positive plants. Data were subjected to Kruskal-Wallis analysis of variance on ranks followed by all pairwise multiple comparison of treatments using the Student-Newman-Keuls method. The differences in transgenic plant formation between the treatments were not statistically significant (P ≤ 0.05)

ISSN: 1746-4811