Genetic transformation of the oilseed crop camelina using immature zygotic embryos

Table 3 Effect of Agrobacterium density, duration of co-cultivation, and Acetosyringone concentration on transformation efficiency

No. of IZEs¹	OD₆₀₀ of Agrobacterium at inoculation	Duration of co-cultivation	Acetosyringone concentration [µM]	No. of regenerated plantlets	No. of PCR- positive plants and efficiency²
60	0.3	48 h	150	22	5^ab (8%)
60	0.3	48 h	300	27	4^b (7%)
60	0.3	72 h	150	37	7^ab (12%)
60	0.6	48 h	150	28	8^a (13%)
60	0.6	48 h	300	41	6^ab (10%)

¹20 immature zygotic embryos (IZEs) of line Cam139 were cultivated per culture dish, with three dishes being used as repetitions per treatment
²PCR was conducted using primers specifically amplifying the GFP transgene, and the transformation efficiency was calculated as GFP-positive plants per number of agro-inoculated explants. Data were subjected to Normality and Equal Variance tests followed by comparison of treatments by Student’s t-test. Exclusively different superscript letters given for two treatments denote a significant difference (P ≤ 0.05)

ISSN: 1746-4811