Genetic transformation of the oilseed crop camelina using immature zygotic embryos

Table 4 Effect of Agrobacterium strains on the transformation efficiency

No. of IZEs¹	Agrobacterium strain	No. of plantlets generated	No. of PCR-positive plants and efficiency²	% PCR-negative plants
60	LBA4404/pSB1	22	7b (12%)	68.2
60	AGL1	27	13a (22%)	51.9

¹20 immature zygotic embryos (IZEs) of line Cam139 were cultivated per culture dish, with three dishes being used as repetitions per treatment
²PCR was performed using primers specifically amplifying the GFP transgene, and the transformation efficiency was calculated as proportion of agro-inoculated explants giving rise to GFP-positive plants. Data were subjected to Kruskal-Wallis analysis of variance on ranks followed by comparison of treatments using Student-Newman-Keuls method. Different superscript letters given for the two treatments denote a significant difference (P ≤ 0.05)

ISSN: 1746-4811