Genetic transformation of the oilseed crop camelina using immature zygotic embryos

Table 6 Test of the transformation method across three camelina genotypes

No. of IZEs¹	Genotype	Hygromycin concentration [mg/L]		No. of plantlets generated	No. of PCR-positive plants and efficiency²	% PCR-negative plants
No. of IZEs¹	Genotype	Shoot initiation I	Shoot initiation II	No. of plantlets generated	No. of PCR-positive plants and efficiency²	% PCR-negative plants
60	Cam139	25	25	27	4 (7%)	85.2
60	Cam139	50	25	37	9 (15%)	75.7
60	Calena	25	25	29	5 (8%)	82.8
60	Calena	50	25	76	8 (13%)	89.5
60	Ligena	25	25	22	6 (10%)	72.7
60	Ligena	50	25	53	10 (17%)	81.1

¹20 immature zygotic embryos (IZEs) were cultivated per culture dish, with 3 dishes being used as repetitions per treatment
²PCR was performed using primers specifically amplifying the GFP transgene, and the transformation efficiency was calculated as GFP-positive plants per number of agro-inoculated explants. Data were subjected to Kruskal-Wallis analysis of analysis of variance on ranks followed by all pairwise multiple comparison of treatments using the Student-Newman-Keuls method. However, the differences in transgenic plant formation between the treatments were not statistically significant (P ≤ 0.05)

ISSN: 1746-4811