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Table 7 Analysis of transgene segregation in T1 siblings of Cv. Ligena via germination on medium supplemented with 40 mg/l hygromycin

From: Genetic transformation of the oilseed crop camelina using immature zygotic embryos

T0 mother plants

Number of T1 plants

analyzed

Hygromycin-resistant vs. susceptible seedlings

Segregation

observed

(expected)

χ2 value

P5

20

11:9

1.2:1 (3:1)

4.3

P52

140

26:114

1:4.4 (3:1)

237.7

P85

40

28:12

2.3:1 (3:1)

0.53*

P126

60

25:35

1:1.4 (3:1)

35.6

  1. The formation of a radicle indicated germination ability of all seeds analyzed. Hygromycin-susceptible (non-transgenic) seedlings ceased their development after the emergence of the radicle
  2. *The asterisk indicates that the segregation obtained does not significantly deviate from the expected (Mendelian) ratio according to the Chi-square test at the significance level of 0.05
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Plant Methods

ISSN: 1746-4811