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Table 8 Analysis of transgene segregation in T1 siblings of Cv. Calena using GFP-specific PCR

From: Genetic transformation of the oilseed crop camelina using immature zygotic embryos

T0 mother plants

Number of T1 siblings

analyzed

Seedlings with GFP-positive vs. negative PCR

Segregation

observed

(expected)

χ2 value

P8

20

16:4

4:1 (3:1)

0.27*

P9a1

20

10:10

1:1 (3:1)

6.67

P9e

20

13:7

1.86:1 (3:1)

1.07*

P21a

20

16:4

4:1 (3:1)

0.27*

P24

20

4:16

1:4 (3:1)

32.27

  1. 1Smaller case letters included in the plant code specify different sister lines derived from the same embryo explant
  2. *Asterisks indicate that the segregation obtained does not significantly deviate from the expected (Mendelian) ratio according to the Chi-square test at the significance level of 0.05
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Plant Methods

ISSN: 1746-4811