Skip to main content

Table 9 Analysis of transgene segregation in T1 siblings of Cv. Ligena based on GFP fluorescence via germination on hygromycin-free medium

From: Genetic transformation of the oilseed crop camelina using immature zygotic embryos

T0 mother plants

No. of T1 seedlings

analyzed

GFP fluorescence-positive vs. negative seedlings

Segregation

observed

(expected)

χ2 value

P5

19

14:5

2.8:1 (3:1)

0.92*

P52

20

6:14

1:2.3 (3:1)

21.6

P85

20

13:7

1.86:1 (3:1)

1.07*

P126

20

7:13

1:2 (3:1)

17.1

  1. *Asterisks indicate that the segregation obtained does not significantly deviate from the expected (Mendelian) ratio according to the Chi-square test at the significance level of 0.05
Back to article page

Plant Methods

ISSN: 1746-4811