Fig. 2

Tracing lateral and adventitious root cell lineages using the pBI101-WOX5_pro-CRE/LOX-GUS system. A–E. GUS staining of the pBI101-WOX5_pro-CRE/LOX-GUS transgenic line, showing positive GUS signals in lateral roots (A), lateral root primordia (B–D), and lateral root meristem (E). Seedlings were germinated and grown vertically on ½ MS DEX-free medium (no DEX) for 7 days (d), then cultured on ½ MS medium containing 10 μM DEX (+ DEX) for 3 days. F. Diagram of adventitious root formation from detached Arabidopsis leaves in (G). G. GUS staining of the pBI101-WOX5_pro-CRE/LOX-GUS transgenic line, showing positive GUS signals in adventitious roots growing from detached leaves. Seedlings were cultured for 12 days on DEX-free ½ MS medium, and then two leaves (first leaf pair) were detached from the same plant. One was cultured on B5 medium with 10 μM DEX (+ DEX) for 7 days, and the other was cultured on B5 medium without DEX (no DEX) for 7 days as the control. Two independent lines were analyzed and showed the same results. Scale bars, 1 mm in (A, G) and 50 μm in (B–E)