Fig. 5
Cell lineage during SAM formation in tissue culture. A, B. Diagram (A) and GUS staining result (B) of SAM regeneration in tissue culture with constant DEX treatment on CIM. Hypocotyl explants from pBI101-WOX5pro-CRE/LOX-GUS transgenic line were cultured on CIM with 10 μM DEX (+ DEX) for 5 days and 30 min to form callus, which was then moved to SIM without DEX (no DEX) for 7 days. C, D. Diagram (C) and GUS staining result (D) of SAM regeneration in tissue culture with transient DEX treatment on CIM. Hypocotyl explants from pBI101-WOX5pro-CRE/LOX-GUS transgenic line were cultured on CIM for 5 days without DEX, moved to CIM with 10 μM DEX for 30 min, then callus was moved to SIM without DEX (no DEX) and cultured for 7 days. Blue asterisks indicate cells with LOX2272 recombination event, showing positive GUS staining signals (A, C). Two independent lines were analyzed and showed the same results. Scale bars, 100 μm in (B, D)