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Fig. 3 | Plant Methods

Fig. 3

From: Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR

Fig. 3

Mutagenesis of the maize NRT1.1 gene family by multiplex CRISPR/Cas9 editing. (A)NRT1.1 A, B and C gene models depicting exons (blue boxes), introns (grey lines) and the target sites for designed guide RNAs (orange boxes). The arrows on the gene models represent the gene-specific primers used for PCR confirmations of heritable edits in T1 plants. (B, C & D) Sanger sequencing results of PCR products amplified using gene specific primers in gene-edited plants (guide RNA targets and PAM sequences are depicted in orange letters and boxes, respectively; red dashes represent deletions, bold blue underlined letters represent insertions)

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Plant Methods

ISSN: 1746-4811