Fig. 2
From: BioCurve Analyzer: a web-based shiny app for analyzing biological response curves
Estimation of ED50 from dose–response curves. A. The comparison of absolute and relative ED50. The relative 50% response and the corresponding relative ED50 values are indicated in dotted lines, while the absolute 50% response and the corresponding absolute ED50 values are shown in dashed lines. B. Parameters estimated from the biphasic curves. The Low/High ED50, LDS (limiting dose for stimulation/inhibition), and M (maximum simulation/inhibition) and the corresponding responses are indicated as dashed lines. C. Reed-and-Muench method in estimating absolute ED50 values. The estimated ED50 and the corresponding response are presented as dashed lines in the figure. The coordinates of the ED50 and the two data points that bracket the ED50 value are shown in the adjacent parentheses, and the function for the linear regression of the two points is displayed. The curves in this figure are generated using simulated data