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Fig. 2 | Plant Methods

Fig. 2

From: Bioluminescent imaging of an oomycete pathogen empowers chemical selections and rational fungicide applications

Fig. 2

High throughput screening of chemical fungicides in 96-well format. a The schematic representation of the high throughput screening in 96-well format. b The bioluminescence result of 96-well plate inhibition assay at day 7. First three lanes display the bioluminescence outcomes of fluazinam, cyazofamid and cymoxanil in four concentrations. The mock lane shows the bioluminescence result of PiLuc + Pea medium, JH19 + Pea medium, Pea medium and ddH2O. c and d Line chart depicting bioluminoscore and inhibition rates of fluazinam, cyazofamid and cymoxanil in four concentrations. X-axis represents fungicides concentration; the y-axis indicates the inhibition rate (%) showing the means ± standard deviation (SD) (n = 3 biological replicates). The inhibition rate was calculated as (integrated signal density PiLuc + PEA medium- integrated signal density chemicals + PEA medium)/integrated signal density PiLuc + PEA medium

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