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  1. D. giganteiformis subsp. pontederae and D. superbus subsp. superbus are protected or critically endangered species in several European regions; therefore, developing an efficient in vitro micropropagation protoco...

    Authors: Dóra Farkas, Judit Csabai, Angéla Kolesnyk, Pál Szarvas and Judit Dobránszki
    Citation: Plant Methods 2025 21:13
  2. Throughout their lifetime, trees store valuable environmental information within their wood. Unlocking this information requires quantitative analysis, in most cases of the surface of wood. The conventional pa...

    Authors: Jan Van den Bulcke, Louis Verschuren, Ruben De Blaere, Simon Vansuyt, Maxime Dekegeleer, Pierre Kibleur, Olivier Pieters, Tom De Mil, Wannes Hubau, Hans Beeckman, Joris Van Acker and Francis wyffels
    Citation: Plant Methods 2025 21:11
  3. Rice blast is one of the most destructive diseases in rice cultivation, significantly threatening global food security. Timely and precise detection of rice panicle blast is crucial for effective disease manag...

    Authors: Shaodan Lin, Deyao Huang, Libin Wu, Zuxin Cheng, Dapeng Ye and Haiyong Weng
    Citation: Plant Methods 2025 21:10
  4. Crossover interactions stemming from phenotypic plasticity complicate selection decisions when evaluating hybrid maize with superior grain yield and consistent performance. Consequently, a two-year, region-wid...

    Authors: Bikas Basnet, Chitra Bahadur Kunwar and Umisha Upreti
    Citation: Plant Methods 2025 21:8
  5. Quantifying plant transpiration via thermal imaging is desirable for applications in agriculture, plant breeding, and plant science. However, thermal imaging under natural non-steady state conditions is curren...

    Authors: Jiayu Zhang, Elias Kaiser, Hanyi Zhang, Leo F. M. Marcelis and Silvere Vialet-Chabrand
    Citation: Plant Methods 2025 21:6
  6. Plant cell walls are made of a complex network of interacting polymers that play a critical role in plant development and responses to environmental changes. Thus, improving plant biomass and fitness requires ...

    Authors: Tasleem Javaid, Akshayaa Venkataraghavan, Matrika Bhattarai, Debkumar Debnath, Wancheng Zhao, Tuo Wang and Ahmed Faik
    Citation: Plant Methods 2025 21:5
  7. Apricot trees, serving as critical agricultural resources, hold a significant role within the agricultural domain. Conventional methods for detecting pests and diseases in these trees are notably labor-intensi...

    Authors: Minglang Li, Zhiyong Tao, Wentao Yan, Sen Lin, Kaihao Feng, Zeyi Zhang and Yurong Jing
    Citation: Plant Methods 2025 21:4
  8. Authors: Jenyne Loarca, Tyr Wiesner-Hanks, Hector Lopez-Moreno, Andrew F. Maule, Michael Liou, Maria Alejandra Torres-Meraz, Luis Diaz-Garcia, Jennifer Johnson-Cicalese, Jeffrey Neyhart, James Polashock, Gina M. Sideli, Christopher F. Strock, Craig T. Beil, Moira J. Sheehan, Massimo Iorizzo, Amaya Atucha…
    Citation: Plant Methods 2025 21:3

    The original article was published in Plant Methods 2024 20:172

  9. The rapid production of doubled haploids by anther culture technology is an important breeding method for awnless triticale. The aim of this study was to explore the effects of triticale genotype and the types...

    Authors: Jun Ma, Fangyuan Zhao, Yinxia Zhang, Xinhui Tian and Wenhua Du
    Citation: Plant Methods 2025 21:2
  10. Virus-induced gene silencing (VIGS) is a rapid and powerful method for gene functional analysis in plants that pose challenges in stable transformation. Numerous VIGS systems based on Agrobacterium infiltration h...

    Authors: Hongjian Shen, Huajie Chen, Weimeng Li, Shan He, Boyong Liao, Wanyu Xiong, Yang Shen, Yongjuan Li, Yanru Gao, Yong Quan Li and Bipei Zhang
    Citation: Plant Methods 2025 21:1
  11. The genus Flaveria has been studied extensively as a model for the evolution of C4 photosynthesis. Thus far, molecular analyses in this genus have been limited due to a dearth of genomic information and the lack ...

    Authors: Christopher J. Baros, Jeremy Beerkens and Martha Ludwig
    Citation: Plant Methods 2024 20:193
  12. Genetic markers are crucial for breeding crops with desired agronomic traits, and their development can be expedited using next-generation sequencing (NGS) and bioinformatics tools. Numerous tools have been de...

    Authors: Joo-Seok Park, Yoram Choi, Jin-Hyun Kim, Chaeyoung Lee, Min-Gyun Jeong, Yeong-Il Jeong, Yang Jae Kang, Young-Soo Chung and Hong-Kyu Choi
    Citation: Plant Methods 2024 20:192
  13. Genetic transformation is a pivotal approach in plant genetic engineering. Peanut (Arachis hypogaea L.) is an important oil and cash crop, but the stable genetic transformation of peanut is still difficult and in...

    Authors: Chen Huang, Chen Yang, Huifang Yang, Yadi Gong, Xiaomeng Li, Lexin Li, Ling Li, Xu Liu and Xiaoyun Li
    Citation: Plant Methods 2024 20:190
  14. This research proposes an easy to apply quality assurance pipeline for hyperspectral imaging (HSI) systems used for plant phenotyping. Furthermore, a concept for the analysis of quality assured hyperspectral i...

    Authors: Justus Detring, Abel Barreto, Anne-Katrin Mahlein and Stefan Paulus
    Citation: Plant Methods 2024 20:189
  15. Senescence is a complex developmental process that is regulated by a multitude of environmental, genetic, and physiological factors. Optimizing the timing and dynamics of this process has the potential to sign...

    Authors: Flavian Tschurr, Lukas Roth, Nicola Storni, Olivia Zumsteg, Achim Walter and Jonas Anderegg
    Citation: Plant Methods 2024 20:188
  16. Gene expression is a fundamental process for plants to express their phenotype, and its analysis is the basis of molecular studies. However, the instability of RNA often poses an obstacle to analyzing plants g...

    Authors: Yoshiaki Ueda
    Citation: Plant Methods 2024 20:187
  17. Verticillium wilt greatly hampers Chinese cabbage growth, causing significant yield limitations. Rapid and accurate detection of Verticillium wilt in the Chinese cabbage (Brassica rapa L. ssp. pekinensis) can pro...

    Authors: Jun Zhang, Dongfang Zhang, Jingyan Liu, Yuhong Zhou, Xiaoshuo Cui and Xiaofei Fan
    Citation: Plant Methods 2024 20:186
  18. Nitrate (NO3−) is one of the two major forms of inorganic nitrogen absorbed by plant roots, and the tissue nitrate concentration in roots is considered important for optimizing developmental programs. Technologie...

    Authors: Alma Fernández González, Ze Tian Fang, Dipankar Sen, Brian Henrich, Yukihiro Nagashima, Alexei V. Sokolov, Sakiko Okumoto and Aart J. Verhoef
    Citation: Plant Methods 2024 20:185
  19. Leaf water content (LWC) encapsulates critical aspects of tree physiology and is considered a proxy for assessing tree drought stress and the risk of forest decline; however, its measurement relies on destruct...

    Authors: Zhao-Kui Li, Hong-Li Li, Xue-Wei Gong, Heng-Fang Wang and Guang-You Hao
    Citation: Plant Methods 2024 20:184
  20. Root growth is most commonly determined with the destructive soil core method, which is very labor-intensive and destroys the plants at the sampling spots. The alternative minirhizotron technique allows for ro...

    Authors: Jessica Arnhold, Facundo R. Ispizua Yamati, Henning Kage, Anne-Katrin Mahlein, Heinz-Josef Koch and Dennis Grunwald
    Citation: Plant Methods 2024 20:183

    The Correction to this article has been published in Plant Methods 2025 21:7

  21. In recent years, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have emerged as critical regulators in plant biology, governing complex gene regulatory networks. In the context of disease resistance in Hev...

    Authors: Yanluo Zeng, Tianbin Guo, Liping Feng, Zhuoda Yin, Hongli Luo and Hongyan Yin
    Citation: Plant Methods 2024 20:182
  22. For the first time, a novel simple label-free electrochemical immunosensor was fabricated for sensitive detection of the coat protein of beet necrotic yellow vein virus (CP-BNYVV) as the causal agent of Rhizom...

    Authors: Marziye Karimzade, Hashem Kazemzadeh-Beneh, Negar Heidari, Mehrasa Rahimi Boroumand, Parviz Norouzi, Mohammad Reza Safarnejad and Masoud Shams-Bakhsh
    Citation: Plant Methods 2024 20:181
  23. Quantifying drought tolerance in crops is critical for agriculture management under environmental change, and drought response traits in grape vine have long been the focus of viticultural research. Turgor los...

    Authors: Adam R. Martin, Guangrui Li, Boya Cui, Rachel O. Mariani, Kale Vicario, Kimberley A. Cathline, Allison Findlay and Gavin Robertson
    Citation: Plant Methods 2024 20:180
  24. Climate change and the growing demand for agricultural water threaten global food security. Understanding water use characteristics of major crops from leaf to field scale is critical, particularly for identif...

    Authors: Daisuke Sugiura, Shiro Mitsuya, Hirokazu Takahashi, Ryo Yamamoto and Yoshiyuki Miyazawa
    Citation: Plant Methods 2024 20:179
  25. Post-harvest physiological deterioration (PPD) poses a significant challenge to the cassava industry, leading to substantial economic losses. This study aims to address this issue by developing a comprehensive...

    Authors: Daniela Gómez Ayalde, Juan Camilo Giraldo Londoño, Audberto Quiroga Mosquera, Jorge Luis Luna Melendez, Winnie Gimode, Thierry Tran, Xiaofei Zhang and Michael Gomez Selvaraj
    Citation: Plant Methods 2024 20:178
  26. The slow breeding cycle presents a significant challenge in legume research and breeding. While current speed breeding (SB) methods promise faster plant turnover, they encounter space limitations and high cost...

    Authors: S. Gurumurthy, Apoorva Ashu, S. Kruthika, Amol P. Solanke, T. Basavaraja, Khela Ram Soren, Jagadish Rane, Himanshu Pathak and P. V. Vara Prasad
    Citation: Plant Methods 2024 20:177
  27. In soybean breeding programs, a great deal of time is devoted to the use of methods that perform selection of individual plants during the initial generations. Our hypothesis is that BLUPIS (simulated individu...

    Authors: Andreia Schuster, Felipe Lopes da Silva, João Amaro Ferreira Vieira Netto, Emanuel Ferrari do Nascimento, Paulo Eduardo Teodoro and Leonardo Lopes Bhering
    Citation: Plant Methods 2024 20:176
  28. The early and specific detection of abiotic and biotic stresses, particularly their combinations, is a major challenge for maintaining and increasing plant productivity in sustainable agriculture under changin...

    Authors: Hans Lukas Bethge, Inga Weisheit, Mauritz Sandro Dortmund, Timm Landes, Miroslav Zabic, Marcus Linde, Thomas Debener and Dag Heinemann
    Citation: Plant Methods 2024 20:175
  29. Peucedanum praeruptorum Dunn has typical stacked umbels and medicinal value; however, the lack of an effective tissue culture system for P. praeruptorum has limited the large-scale propagation of its seedlings.

    Authors: Haoyu Pan, Ranran Liao, Yingyu Zhang, Muhammad Arif, Yuxin Zhang, Shuai Zhang, Yuanyuan Wang, Pengcheng Zhao, Zaigui Wang, Bangxing Han and Cheng Song
    Citation: Plant Methods 2024 20:174
  30. As one of the world’s most important vegetable crops, eggplant production is often severely affected by verticillium wilt, leading to significant declines in yield and quality. Traditional multispectral diseas...

    Authors: Youwei Zhang, Dongfang Zhang, Yunfei Zhang, Fengqing Cheng, Xuming Zhao, Min Wang and Xiaofei Fan
    Citation: Plant Methods 2024 20:173
  31. BerryPortraits (Phenotyping of Ripening Traits) is open source Python-based image-analysis software that rapidly detects and segments berries and extracts morphometric data on fruit quality traits such as berr...

    Authors: Jenyne Loarca, Tyr Wiesner-Hanks, Hector Lopez-Moreno, Andrew F. Maule, Michael Liou, Maria Alejandra Torres-Meraz, Luis Diaz-Garcia, Jennifer Johnson-Cicalese, Jeffrey Neyhart, James Polashock, Gina M. Sideli, Christopher F. Strock, Craig T. Beil, Moira J. Sheehan, Massimo Iorizzo, Amaya Atucha…
    Citation: Plant Methods 2024 20:172

    The Correction to this article has been published in Plant Methods 2025 21:3

  32. Understanding the environmental impacts on root growth and root health is essential for effective agricultural and environmental management. Hyperspectral imaging (HSI) technology provides a non-destructive me...

    Authors: Corine Faehn, Grzegorz Konert, Markku Keinänen, Katja Karppinen and Kirsten Krause
    Citation: Plant Methods 2024 20:171
  33. Quantitative disease resistance (QR) is a complex, dynamic trait that is most reliably quantified in field-grown crops. Traditional disease assessments offer limited potential to disentangle the contributions ...

    Authors: Jonas Anderegg, Radek Zenkl, Norbert Kirchgessner, Andreas Hund, Achim Walter and Bruce A. McDonald
    Citation: Plant Methods 2024 20:170
  34. Foxtail millet is an important minor cereal crop rich in nutrients. Due to the small size of its seeds, there is little information on the diversity of its seed structure among germplasms, limiting the identif...

    Authors: Yuyuan Miao, Rongxia Wang, Zejun Jing, Kun Wang, Meixia Tan, Fuzhong Li, Wuping Zhang, Jiwan Han and Yuanhuai Han
    Citation: Plant Methods 2024 20:169
  35. Foxtail millet [Setaria italica (L.) Beauv] is a C4 graminoid crop cultivated mainly in the arid and semiarid regions of China for more than 7000 years. Its grain highly nutritious and is rich in starch, protein,...

    Authors: Zhenyu Wang, Jiongyu Hao, Xiaofan Shi, Qiaoqiao Wang, Wuping Zhang, Fuzhong Li, Luis A. J. Mur, Yuanhuai Han, Siyu Hou, Jiwan Han and Zhaoxia Sun
    Citation: Plant Methods 2024 20:168
  36. With the emergence of the new generation vision architecture Vmamba and the further demand for agricultural yield and efficiency, we propose an efficient and high-accuracy target detection network for automate...

    Authors: Peirui Zhao, Weiwei Cai, Wenhua Zhou and Na Li
    Citation: Plant Methods 2024 20:167
  37. Cucumis melo L., commonly known as melon, is a crucial horticultural crop. The selection and breeding of superior melon germplasm resources play a pivotal role in enhancing its marketability. However, current met...

    Authors: Shan Xu, Jia Shen, Yuzhen Wei, Yu Li, Yong He, Hui Hu and Xuping Feng
    Citation: Plant Methods 2024 20:166
  38. Nanomaterial-mediated plant genetic engineering holds promise for developing new crop cultivars but can be hindered by nanomaterial toxicity to protoplasts. We present a fast, high-throughput method for assess...

    Authors: Zhila Osmani, Muhammad Amirul Islam, Feng Wang, Sabrina Rodrigues Meira and Marianna Kulka
    Citation: Plant Methods 2024 20:165
  39. Building models that allow phenotypic evaluation of complex agronomic traits in crops of global economic interest, such as grain yield (GY) in soybean and maize, is essential for improving the efficiency of br...

    Authors: Paulo E. Teodoro, Larissa P. R. Teodoro, Fabio H. R. Baio, Carlos A. Silva Junior, Dthenifer C. Santana and Leonardo L. Bhering
    Citation: Plant Methods 2024 20:164
  40. In spatio-temporal plant monitoring, optical sensing (including hyperspectral imaging), is being deployed to, non-invasively, detect and diagnose plant responses to abiotic and biotic stressors. Early and accu...

    Authors: Christian Nansen, Patrice J. Savi and Anil Mantri
    Citation: Plant Methods 2024 20:163
  41. The use of renewable energy for sustainable and climate-neutral electricity production is increasing worldwide. High-voltage direct-current (HVDC) transmission via underground cables helps connect large produc...

    Authors: Ken Uhlig, Jan Rücknagel and Janna Macholdt
    Citation: Plant Methods 2024 20:162
  42. The cotton whitefly (Bemisia tabaci) is a major global pest, causing significant crop damage through viral infestation and feeding. Traditional B. tabaci recognition relies on human eyes, which requires a large a...

    Authors: Zhen Feng, Nan Wang, Ying Jin, Haijuan Cao, Xia Huang, Shuhan Wen and Mingquan Ding
    Citation: Plant Methods 2024 20:161
  43. Plant breeding played a very important role in transforming strawberries from being a niche crop with a small geographical footprint into an economically important crop grown across the planet. But even modern...

    Authors: Esther Rosales Sanchez, R. Jordan Price, Federico Marangelli, Kirsty McLeary, Richard J. Harrison and Anindya Kundu
    Citation: Plant Methods 2024 20:160

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