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  1. Pest infestations in rice crops greatly affect yield and quality, making early detection essential. As most rice pests affect leaves and rhizomes, visual inspection of rice for pests is becoming increasingly i...

    Authors: Jinsheng Wang, Tao Wang, Qin Xu, Lu Gao, Guosong Gu, Liangquan Jia and Chong Yao
    Citation: Plant Methods 2025 21:63
  2. Sunflowers (Helianthus annuus L.), a vital oil crop, are facing a severe challenge from broomrape (Orobanche cumana), a parasitic plant that seriously jeopardizes the growth and development of sunflowers, limits ...

    Authors: Yingxue Che, Congzi Zhang, Jixiang Xing, Qilemuge Xi, Ying Shao, Lingmin Zhao, Shuchun Guo and Yongchun Zuo
    Citation: Plant Methods 2025 21:62
  3. Simple sequence repeats (SSRs) are widely used as molecular markers; however, traditional development of SSR molecular markers heavily relies on experimental methods. The advancement of modern sequencing techn...

    Authors: Chenhao Jiang, Chuan Dong, Zhenzhen Wu, Chenyi Shi, Qiannan Ye, Xiaopei Wu, Siyi Ma, Yuming Wen, Guoping Yu, Jiasheng Wu and Chengjun Zhang
    Citation: Plant Methods 2025 21:61
  4. Productivity and fitness of cultivated plants are influenced by genetic heritage and environmental interactions, shaping certain phenotypes. Phenomics is the -omics methodology providing applicative approaches...

    Authors: Alice Checcucci, Francesca Decorosi, Giulia Alfreducci, Roberto Natale, Agnese Bellabarba, Stefano Biricolti, Donatella Paffetti, Alessio Mengoni and Carlo Viti
    Citation: Plant Methods 2025 21:58
  5. Fungicides play an indispensable role in ensuring food security. However, rational chemical selection and fungicide precision application guidance remain constrained by the limitations in real-time monitoring ...

    Authors: Han Chen, Jiana Mao, Yujie Fang, Waqas Raza, Zhi Li, Chongyuan Zhang, Yingguang Zhu, Yuanchao Wang and Suomeng Dong
    Citation: Plant Methods 2025 21:57
  6. Water lily (Nymphaea micrantha Guill. & Perr) is an aquatic plant that is well known for its nutritional value and medicinal uses. The lack of adequate information regarding propagation and farming techniques has...

    Authors: Fei Lin, Yong Kang, Arwa Abdulkreem AL-Huqail, Dikhnah Alshehri, Yamei Li, Yuhua Guo, Guangsui Yang, Fenling Tang, Junmei Yin, Zheli Ding and Mamdouh A. Eissa
    Citation: Plant Methods 2025 21:56
  7. Rapeseed (Brassica napus L.) inflorescence coverage is a crucial phenotypic parameter for assessing crop growth and estimating yield. Accurate crop cover assessment is typically performed using Unmanned Aerial Ve...

    Authors: Jie Li, Chengyong Zhu, Chenbo Yang, Quan Zheng, Binhui Wang, Jingmin Tu, Qian Zhang, Sheng Liu, Xinfa Wang and Jiangwei Qiao
    Citation: Plant Methods 2025 21:54
  8. Pest management is essential for agricultural production and food security, as pests can cause significant crop losses and economic impact. Early pest detection is key to timely intervention. While object dete...

    Authors: Rui Fu, Shiyu Wang, Mingqiu Dong, Hao Sun, Mohammed Abdulhakim Al-Absi, Kaijie Zhang, Qian Chen, Liqun Xiao, Xuewei Wang and Ye Li
    Citation: Plant Methods 2025 21:53
  9. Plant diseases adversely affect the agricultural sector by substantially affecting food security and limiting production. We introduce PlantCareNet, a novel, automated, end-to-end diagnostic system for plant d...

    Authors: Muhaiminul Islam, AKM Azad, Shifat E. Arman, Salem A. Alyami and Md Mehedi Hasan
    Citation: Plant Methods 2025 21:52
  10. Posture is a critical phenotypic trait that reflects crop growth and serves as an essential indicator for both agricultural production and scientific research. Accurate pose estimation enables real-time tracki...

    Authors: Yuchen Pan, Jianye Chang, Zhemeng Dong, Bingwen Liu, Li Wang, Hailin Liu and Jue Ruan
    Citation: Plant Methods 2025 21:51
  11. Identifying mutant traits is essential for improving crop yield, quality, and stress resistance in plant breeding. Historically, the efficiency of breeding has been constrained by throughput and accuracy. Rece...

    Authors: Zhe Li, Jinhu Mu, Yan Du, Xiao Liu, Lixia Yu, Jianing Ding, Jing Long, Jingmin Chen and Libin Zhou
    Citation: Plant Methods 2025 21:50
  12. The analysis of translationally active mRNAs, or translatome, is a useful approach for monitoring cellular and plant physiological responses. One such method is the translating ribosome affinity purification (...

    Authors: James N. Culver, Meinhart Vallar, Erik Burchard, Sophie Kamens, Sebastien Lair, Yiping Qi, Tamara D. Collum, Christopher Dardick, Choaa A. El-Mohtar and Elizabeth E. Rogers
    Citation: Plant Methods 2025 21:49
  13. Accurately evaluating the water status of walnuts in different growth stages is fundamental to implementing deficit irrigation strategies and improving the yield of walnuts. The crop water stress index (CWSI) ...

    Authors: Lian Mao, Sen Lu, Linqi Liu, Zhipeng Li, Baoqing Wang, Dong Pei and Yongchao Bai
    Citation: Plant Methods 2025 21:47
  14. Deep learning-based computer vision technology significantly improves the accuracy and efficiency of crop disease detection. However, the scarcity of crop disease images leads to insufficient training data, li...

    Authors: Chao Wang, Yuting Xia, Lunlong Xia, Qingyong Wang and Lichuan Gu
    Citation: Plant Methods 2025 21:46
  15. Smooth bromegrass (Bromus inermis) was adopted as experiment materials for identifying the seed maturity using a combination of multispectral imaging and machine learning. The trials were conducted to investigate...

    Authors: Chengming Ou, Zhicheng Jia, Shiqiang Zhao, Shoujiang Sun, Ming Sun, Jingyu Liu, Manli Li, Shangang Jia and Peisheng Mao
    Citation: Plant Methods 2025 21:45
  16. Seed testing plays a crucial role in improving crop yields.In actual seed testing processes, factors such as grain sticking and complex imaging environments can significantly affect the accuracy of wheat grain...

    Authors: Xin Wang, Changchun Li, Chenyi Zhao, Yinghua Jiao, Hengmao Xiang, Xifang Wu and Huabin Chai
    Citation: Plant Methods 2025 21:44
  17. CRISPR/Cas9 based genome editing has advanced our understanding of a myriad of important biological phenomena. Important challenges to multiplex genome editing in maize include assembly of large complex DNA co...

    Authors: Maruti Nandan Rai, Brian Rhodes, Stephen Jinga, Praveena Kanchupati, Edward Ross, Shawn R. Carlson and Stephen P. Moose
    Citation: Plant Methods 2025 21:43
  18. Accurate and efficient assessment of highland barley (Hordeum vulgare L.) density is crucial for optimizing cultivation and management practices. However, challenges such as overlapping spikes in unmanned aerial ...

    Authors: Minghui Cai, Hui Deng, Jianwei Cai, Weipeng Guo, Zhipeng Hu, Dongzheng Yu and Houxi Zhang
    Citation: Plant Methods 2025 21:42
  19. Hybrid breeding, a direct and efficient strategy for disease control and management in tree species, is currently limited by the selection method of resist clones: the “in vitro stem segment inoculation method...

    Authors: Zheng Li, Bingyu Zhang, Yuchen Fu, Yutian Suo, Yinan Zhang, Jinxia Feng, Long Pan, Wanna Shen, Huixiang Liu, Xiaohua Su and Jiaping Zhao
    Citation: Plant Methods 2025 21:41
  20. In the process of smart breeding, the rapid statistics of soybean emergence rate, as an important part of breeding screening, face challenges under environmental constraints, especially the selection and breed...

    Authors: Yan Sun, Mengqi Li, Meiling Liu, Jingyi Zhang, Yingli Cao and Xue Ao
    Citation: Plant Methods 2025 21:40
  21. The expected grain yield response to plant density in winter wheat (Triticum aestivum L.) follows a diminishing returns function. To our knowledge, all previous studies dealing with plant density have assumed con...

    Authors: Nicolas Giordano, Dustin Hayes, Trevor J. Hefley, Josefina Lacasa, Brian L. Beres, Lucas A. Haag and Romulo P. Lollato
    Citation: Plant Methods 2025 21:38
  22. Cotton phenomics plays a crucial role in understanding and managing the growth and development of cotton plants. The segmentation of point clouds, a process that underpins the measurement of plant organ struct...

    Authors: Fu-Yong Liu, Hui Geng, Lin-Yuan Shang, Chun-Jing Si and Shi-Quan Shen
    Citation: Plant Methods 2025 21:37
  23. The root apical meristem (RAM) and the shoot apical meristem (SAM), which contain stem cells and their lineages, are the centers for underground and aboveground organ formation in vascular plants. Accurately t...

    Authors: Shengnan Wang, Yu Li, Zijuan Li, Chao Li, Lin Xu and Ning Zhai
    Citation: Plant Methods 2025 21:36
  24. Camelina is an oilseed crop with particularly useful fatty acid and amino acid profiles of its seeds, high resilience to abiotic and biotic stresses, and a short life cycle. Previous genetic engineering approa...

    Authors: Barno Ruzimurodovna Rezaeva, Ingrid Otto, Götz Hensel, Pouneh Pouramini, Anton Peterson and Jochen Kumlehn
    Citation: Plant Methods 2025 21:35
  25. Studies of multicopy genes impose challenges related to gene redundancy and sequence similarity among copies. However, recent advances in molecular biology and genomics tools associated with dedicated database...

    Authors: Vania Gabriela Sedano Partida, Henrique Moura Dias, Maria Teresa Portes and Marie-Anne Van Sluys
    Citation: Plant Methods 2025 21:32
  26. European beech (Fagus sylvatica L.) is distributed across diverse climate conditions throughout Europe. Local adaptations, such as drought tolerance, could become crucial for maintaining beech populations facing ...

    Authors: Virginia Zahn, Alexander Fendel, Alice-Jeannine Sievers, Matthias Fladung and Tobias Bruegmann
    Citation: Plant Methods 2025 21:31
  27. There is much interest in how roots can be manipulated to improve crop performance in a changing climate, yet root research is made difficult by the challenges of visualising the root system accurately, partic...

    Authors: Cara Wharton, Andrew Beacham, Miriam L. Gifford and James Monaghan
    Citation: Plant Methods 2025 21:29
  28. Pollination strategies to supplement or replace insect pollinators are needed to produce marketable strawberry fruits in indoor vertical farms. To ensure the self-pollination of strawberry flowers, anther dehi...

    Authors: Hyein Lee, Meiyan Cui, Byungkwan Lee, Jeesang Myung, Jaewook Shin and Changhoo Chun
    Citation: Plant Methods 2025 21:28
  29. Monitoring plant growth is crucial for effective crop management, and using color and depth (RGBD) cameras to model lettuce has emerged as one of the most convenient and non-invasive methods. In recent years, ...

    Authors: Victor Bloch, Alexey Shapiguzov, Titta Kotilainen and Matti Pastell
    Citation: Plant Methods 2025 21:27
  30. The chlorophyll content has a strong influence on plant photosynthesis and crop growth and is a key factor for understanding the functioning of farming systems. Therefore, the accurate estimation of chlorophyl...

    Authors: Honggang Zhang, Dan Zhao, Zhonghui Guo, Sien Guo, Quchi Bai, Huini Cao, Shuai Feng, Fenghua Yu and Tongyu Xu
    Citation: Plant Methods 2025 21:26
  31. The enhancement of rice quality stands as a pivotal focus in crop breeding research, with spectral analysis-based non-destructive quality assessment emerging as a widely adopted tool in agriculture. A prevalen...

    Authors: Jing Guo, Sijia Jiang, Bingjie Lu, Wei Zhang, Yinyin Zhang, Xiao Hu, Wanneng Yang, Hui Feng and Liang Xu
    Citation: Plant Methods 2025 21:25
  32. Digital color indices provide a reliable means for assessing plant status by enabling real-time estimation of chlorophyll (Chl) content, and are thus adopted widely for crop monitoring. However, as all prevale...

    Authors: Avinash Agarwal, Filipe de Jesus Colwell, Viviana Andrea Correa Galvis, Tom R. Hill, Neil Boonham and Ankush Prashar
    Citation: Plant Methods 2025 21:24

    The Correction to this article has been published in Plant Methods 2025 21:59

  33. The production of virus-free apricots (Prunus armeniaca L.) is essential for controlling viral diseases, exchanging breeding materials without the risk of spreading new diseases, and preserving plant germplasm. P...

    Authors: C. Pérez-Caselles, L. Burgos, E. Yelo, L. Faize and N. Alburquerque
    Citation: Plant Methods 2025 21:23
  34. Late and early leaf spot in peanuts is a foliar disease contributing to a significant amount of lost yield globally. Peanut breeding programs frequently focus on developing disease-resistant peanut genotypes. ...

    Authors: Joshua Larsen, Jeffrey Dunne, Robert Austin, Cassondra Newman and Michael Kudenov
    Citation: Plant Methods 2025 21:22
  35. Accurate detection of missed tassels is crucial for maintaining the purity of hybrid maize seed production. This study introduces the MT-YOLO model, designed to replace or assist manual detection by leveraging...

    Authors: Jiangtao Qi, Chenchen Ding, Ruirui Zhang, Yuxin Xie, Longlong Li, Weirong Zhang and Liping Chen
    Citation: Plant Methods 2025 21:21
  36. Monitoring sorghum during the flowering stage is essential for effective fertilization management and improving yield quality, with spike identification serving as the core component of this process. Factors s...

    Authors: Song Zhang, Yehua Yang, Lei Tu, Tianling Fu, Shenxi Chen, Fulang Cen, Sanwei Yang, Quanzhi Zhao, Zhenran Gao and Tengbing He
    Citation: Plant Methods 2025 21:20
  37. The high productivity of Eucalyptus spp. forest plantations is mainly due to advances in silvicultural techniques and genetic improvement associated with the potential that many species of the genus have for vege...

    Authors: Thiago Romanos Benatti, Filipe Manoel Ferreira, Rodolfo Manoel Lemes da Costa, Mario Luiz Teixeira de Moraes, Aurélio Mendes Aguiar, Donizete da Costa Dias, José Wilacildo de Matos, Aline Cristina Miranda Fernandes, Mateus Chagas Andrade, Leandro de Siqueira, Itaraju Junior Baracuhy Brum, André Vieira do Nascimento, Yuri Tani Utsunomiya, José Fernando Garcia and Evandro Vagner Tambarussi
    Citation: Plant Methods 2025 21:19
  38. The problem of plant diseases is huge as it affects the crop quality and leads to reduced crop production. Crop-Convolutional neural network (CNN) depiction is that several scholars have used the approaches of...

    Authors: B. V. Baiju, Nancy Kirupanithi, Saravanan Srinivasan, Anjali Kapoor, Sandeep Kumar Mathivanan and Mohd Asif Shah
    Citation: Plant Methods 2025 21:18
  39. Shapefiles are a geospatial vector data format used to indicate geographic features in geographic information systems (GIS) software. Shapefiles are used in high-throughput phenotyping plant breeding and agron...

    Authors: Nathaniel Burner, Donna K. Harris and Zenglu Li
    Citation: Plant Methods 2025 21:17
  40. In the era of machine learning-driven plant imaging, the production of annotated datasets is a very important contribution. In this data paper, a unique annotated dataset of seedling emergence kinetics is prop...

    Authors: Félix Mercier, Geoffroy Couasnet, Angelina El Ghaziri, Nizar Bouhlel, Alain Sarniguet, Muriel Marchi, Matthieu Barret and David Rousseau
    Citation: Plant Methods 2025 21:16
  41. Hyperspectral imaging combined with machine learning offers a promising, cost-effective alternative to invasive chemical analysis for early plant disease detection. In this study, the use of 3D Convolutional N...

    Authors: Rizos-Theodoros Chadoulis, Ioannis Livieratos, Ioannis Manakos, Theodore Spanos, Zeinab Marouni, Christos Kalogeropoulos and Constantine Kotropoulos
    Citation: Plant Methods 2025 21:15

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